An overview of LC –MS applications, including food, environmental, industrial, GPC, and biopharmaceutical analysis
Concepts, perspectives, best practices, and potential issues surrounding UHPLC
Fundamentals of LC-MS method development and easier approaches,Overview of method validation and transferStandard operating procedures for LC-MS modules, and troubleshooting strategies.
Theory of Single Quadrupole, Triple Quadrupole, System overview & Ionization sources, detectors, Collision induced Dissociation, Solvents, buffers & additives used in LC-MS. MS operation, including the operation of the most popular LC-MS interfaces
Operation in MS, MS/MS, and MS/MS/MS modes. Tuning and Calibration, Product Ion Scan, MRM Method and HPLC Method. Ion production, fragmentation, and detection,MS calibration and optimization. LC-MS Sample Preparation for Pesticide Analysis, LC –MS Sample Preparation for analysis of Organic pollutants,Developing calibration curve.
Method optimizations and the validations of parameters such as LOD, LOQ, accuracy, precision, linearity and robustness in LC-MS-MS. Sample analysis of Pesticide residue in Water samples, Quantitative analysis of Organic pollutants. Quantitative data analysis with set files,Quantitation using internal standards.
Maintenance and Troubleshooting – Effectively detecting, troubleshooting and rectifying common issues – Performing instrument maintenance Carrying out relevant diagnostic tests – Experience from hands-on laboratory exercises.
Concepts, perspectives, best practices, and potential issues surrounding UHPLC
Fundamentals of LC-MS method development and easier approaches,Overview of method validation and transferStandard operating procedures for LC-MS modules, and troubleshooting strategies.
Theory of Single Quadrupole, Triple Quadrupole, System overview & Ionization sources, detectors, Collision induced Dissociation, Solvents, buffers & additives used in LC-MS. MS operation, including the operation of the most popular LC-MS interfaces
Operation in MS, MS/MS, and MS/MS/MS modes. Tuning and Calibration, Product Ion Scan, MRM Method and HPLC Method. Ion production, fragmentation, and detection,MS calibration and optimization. LC-MS Sample Preparation for Pesticide Analysis, LC –MS Sample Preparation for analysis of Organic pollutants,Developing calibration curve.
Method optimizations and the validations of parameters such as LOD, LOQ, accuracy, precision, linearity and robustness in LC-MS-MS. Sample analysis of Pesticide residue in Water samples, Quantitative analysis of Organic pollutants. Quantitative data analysis with set files,Quantitation using internal standards.
Maintenance and Troubleshooting – Effectively detecting, troubleshooting and rectifying common issues – Performing instrument maintenance Carrying out relevant diagnostic tests – Experience from hands-on laboratory exercises.
