Basic PCR & real time PCR theory Applications & possibilities of qPCR vs traditional endpoint PCR
Review of different of availability detection technologies (SYBR) Green I Taqman, Molecular Beacons
Review of different instrument platforms and their typical uses. Experiments demonstrating basic quantification strategy
The primer-dimer problem and how to minimize it • Probe design of TaqMan and Molecular Beacons • Experimental design
Optimization of PCR
Review of different of availability detection technologies (SYBR) Green I Taqman, Molecular Beacons
Review of different instrument platforms and their typical uses. Experiments demonstrating basic quantification strategy
The primer-dimer problem and how to minimize it • Probe design of TaqMan and Molecular Beacons • Experimental design
Optimization of PCR
