It is estimated that 30% of diseases caused by mutations can be linked to splicing. Mutation of a splice site can result in site loss of function and specificity, causing insertion or deletion of aminoacids or a disruption of the reading frame. The importance of understanding and properly decoding roles governing splicing have become a fact for the research community as demonstarted by the increasing number of manuscripts devoted to this subject. Splicing 2021 intends to join in a friendly academic environment all of us working or interested in moving to this area of research. The conference will take place in summer in one of the best beach areas from Portugal, the sea village of Caparica (South Lisbon area). We aim you to present your latest results in Splicing and to join the best researchers in this area of knowledge. Please do not hesitate in contacting me should you have any suggestion to help us make of this event a remarkable one.. The conference cover Plenary (invited), Keynotes(invited), Caparica Talks (invited), and Welcome Talks as well as shotgun and poster presentations for young researchers.