From Sample Prep, HPLC/UHPLC to MS Instrumentation
Part I. Sample Preparation in LC-MS :
Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:
Part I. Sample Preparation in LC-MS :
- The role/benefits of sample preparation in good LC-MS practice
- Overviews of different sample preparation techniques and their applicability
- Guide to selecting the right Sample Preparation method
- What is the role of the LC column in LC-MS?
- Why is it even necessary?
- Basics of LC theory
- Review of the different LC media/support particles
- Survey of different LC bonded phases and their application
- Guide to selecting the right LC column for your application
Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:
- Atmospheric Pressure Ionisation theory, techniques and methods:
- Desolvation
- Electrospray Ionisation (ESI)
- Atmospheric Chemical Ionisation (APCI)
- Mass Analysis
- The importance of calibration, and the differences between nominal mass, accurate mass and mass resolution
- Mass Analysers
- Quadrupole – benefits, limitations and general usage
- Time of Flight – benefits, limitations and general usage
- Data analysis
- Quantitation
- Application of targeted techniques eg. MRM to data acquisition and processing
- Qualitative approaches to identification
- Application of discovery techniques eg. scanning, information dependent acquisition, library searching
